How to use glutaraldehyde and precautions

- Aug 16, 2018-


Sterilization: Only one method of soaking.

Immerse the items to be sterilized by washing, drying, and immersed in 2% glutaraldehyde solution, cover, soak for 10 hours, remove aseptically, rinse with sterile water, and dry until sterile. Glutaraldehyde was used for 14 days. Disinfection treatment: soaking method. The sterilized articles were immersed in a 2% glutaraldehyde solution and capped. Generally, it is contaminated by bacterial propagules, soaked for 10 minutes, soaked with hepatitis virus for 30 minutes, washed out and rinsed with sterile distilled water and dried. Wiping method: Wipe the surface contaminated by bacterial propagule with 2% glutaraldehyde solution, disinfection for 10 min, hepatitis virus contaminated surface, disinfection for 30 min.


1 2% acid glutaraldehyde is corrosive to metals; 2% neutral glutaraldehyde is corrosive to carbon steel products such as surgical blades. 0.5% sodium nitrite should be added to prevent rust before use.

2 The bactericidal effect of glutaraldehyde is greatly affected by pH. When soaking medical equipment with acid or fortified acid glutaraldehyde, it should be used first.

3% sodium bicarbonate was adjusted to pH 7.5-8.8. When the pH exceeds 9.0, the rapid polymerization of glutaraldehyde loses the bactericidal ability.

32% alkaline glutaraldehyde can only be stored for 2 weeks at room temperature, and the remaining dosage forms can be stored for 4 weeks.

4 Glutaraldehyde is irritating to the skin and mucous membranes. Wear gloves when touching the solution to prevent splashing into the eyes or inhaling the body.

5 To prepare glutaraldehyde, use distilled water, and the container containing glutaraldehyde solution should be clean.

6 Equipment that has been sterilized or sterilized with glutaraldehyde must be thoroughly rinsed with sterile distilled water before use.